Typical technique Ion chromatography

metrohm 850 ion chromatography system

a sample introduced, either manually or autosampler, sample loop of known volume. buffered aqueous solution known mobile phase carries sample loop onto column contains form of stationary phase material. typically resin or gel matrix consisting of agarose or cellulose beads covalently bonded charged functional groups. equilibration of stationary phase needed in order obtain desired charge of column. if column not equilibrated desired molecule may not bind column. target analytes (anions or cations) retained on stationary phase can eluted increasing concentration of charged species displaces analyte ions stationary phase. example, in cation exchange chromatography, positively charged analyte can displaced adding positively charged sodium ions. analytes of interest must detected means, typically conductivity or uv/visible light absorbance.

control ic system requires chromatography data system (cds). in addition ic systems, of these cdss can control gas chromatography (gc) , hplc.